Matsuno N, Inoko H, Ando A, Nakatsuji T, Sato T, Itchikawa S, Sonoda T, Tsuji K
Department of Transplantation, Tokai University School of Medicine, Kanagawa, Japan.
Transplantation. 1990 Jan;49(1):208-12. doi: 10.1097/00007890-199001000-00046.
Southern blot hybridization was performed in 16 pairs of living-related kidney transplant patients and donors, using DNA samples extracted from peripheral blood lymphocytes. The number of HLA-DNA-mismatched bands was used as an indicator for graft survival. The total number of DNA-mismatched bands seemed to be a valuable parameter. This was further analyzed and it was found that DQB in particular could be used to predict graft survival. Especially, usefulness of HLA-DNA typing was found in positive MLR, where good prognosis was strongly related to DQB matching. This method can therefore be applied in selecting a suitable living-related donor with the best chance of graft survival.
对16对亲属活体肾移植患者及其供体进行了Southern印迹杂交，使用从外周血淋巴细胞中提取的DNA样本。HLA-DNA错配条带的数量被用作移植物存活的指标。DNA错配条带的总数似乎是一个有价值的参数。进一步分析发现，尤其是DQB可用于预测移植物存活。特别是，在阳性混合淋巴细胞反应中发现HLA-DNA分型有用，其中良好的预后与DQB匹配密切相关。因此，该方法可用于选择具有最佳移植物存活机会的合适亲属活体供体。
