Fukuda Y, Kimura A, Hoshino S, Tashiro H, Furukawa M, Shintaku S, Hori H, Sasazuki T, Dohi K
Second Department of Surgery, Hiroshima University School of Medicine, Japan.
Tissue Antigens. 1995 Jan;45(1):49-56. doi: 10.1111/j.1399-0039.1995.tb02414.x.
Sixty-five living related kidney transplant pairs were analyzed for matching at HLA class II loci by the polymerase chain reaction (PCR)--sequence specific oligonucleotide probe (SSOP) method. The retrospective HLA matching study revealed that there were many early graft loss cases despite the DQB compatibility, contrary to our expectation. There were 54 DRB1 one-mismatched cases, in which 7 of the 11 (64%) DQB zero-mismatched cases had lost their grafts, while the graft loss cases were only 10 of the 43 (23%) DQB one-mismatched pairs (P value = 0.0006). The DQB matching of these cases was studied in detail, because the PCR-SSOP methods are based on the detection of sequence polymorphisms in a relatively narrow range, i.e., recognized sequences by SSOPs. The PCR--heteroduplex--polymorphism analysis method was developed to analyze the polymorphism in exon 2 of the DQB1 gene. However, all the pairs proved to be compatible for the DQB, demonstrating that the DQB compatibility was associated with a harmful influence on the graft outcome. These observations suggested that the DQB1 incompatibility might exert the low responsiveness to HLA haplo-identical allogeneic transplants.
通过聚合酶链反应（PCR）-序列特异性寡核苷酸探针（SSOP）方法，对65对活体亲属肾移植供受者进行了HLA II类基因座配型分析。回顾性HLA配型研究显示，尽管DQB配型相符，但仍有许多早期移植物丢失病例，这与我们的预期相反。有54例DRB1单错配病例，其中11例DQB零错配病例中有7例（64%）移植物丢失，而在43例DQB单错配供受者对中，移植物丢失病例仅10例（23%）（P值 = 0.0006）。对这些病例的DQB配型进行了详细研究，因为PCR-SSOP方法是基于在相对较窄范围内检测序列多态性，即SSOP识别的序列。开发了PCR-异源双链-多态性分析方法来分析DQB1基因第2外显子的多态性。然而，所有供受者对的DQB均显示相符，这表明DQB相符对移植物结局有不良影响。这些观察结果提示，DQB1不相容可能导致对HLA单倍型相合同种异体移植的低反应性。
