Department of Neuroscience, Mount Sinai School of Medicine, New York, New York, USA.
Nat Protoc. 2011 Mar;6(3):314-26. doi: 10.1038/nprot.2010.193. Epub 2011 Feb 17.
This protocol describes a method for efficient chemical synthesis of dinitroindolinyl derivatives of glutamate and γ-aminobutyric acid. These caged neurotransmitters are currently the most chemically and photochemically efficient probes for two-photon photolysis in living brain slices. The protocol only requires basic organic synthesis equipment, and no silica gel column chromatography or NMR spectroscopy is needed at any stage. HPLC is used to purify the caged transmitters at the end of the syntheses. Thus, the synthesis of dinitroindolinyl-caged neurotransmitters is within the scope of a modestly equipped chemistry laboratory.
本方案描述了一种高效化学合成谷氨酸和γ-氨基丁酸的二硝基吲哚啉衍生物的方法。这些笼状神经递质是目前用于活体脑切片双光子光解的最具化学和光化学效率的探针。该方案仅需要基本的有机合成设备,并且在任何阶段都不需要硅胶柱层析或 NMR 光谱学。在合成结束时,使用 HPLC 来纯化笼状递质。因此,二硝基吲哚啉笼状神经递质的合成在配备适度的化学实验室范围内。