Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 003-1133, Japan, and Center for NanoBio Integration, University of Tokyo, Tokyo, Japan.
Department of Pharmacology and Physiology, Drexel University College of Medicine, Philadelphia, PA 19102, USA.
Nat Chem Biol. 2010 Apr;6(4):255-257. doi: 10.1038/nchembio.321. Epub 2010 Feb 21.
We have synthesized a photosensitive (or caged) 4-carboxymethoxy-5,7-dinitroindolinyl (CDNI) derivative of gamma-aminobutyric acid (GABA). Two-photon excitation of CDNI-GABA produced rapid activation of GABAergic currents in neurons in brain slices with an axial resolution of approximately 2 mum and enabled high-resolution functional mapping of GABA-A receptors. Two-photon uncaging of GABA, the main inhibitory neurotransmitter, should allow detailed studies of receptor function and synaptic integration with subcellular precision.
我们合成了一种对光敏感（或笼状）的 4-羧甲氧-5,7-二硝基吲哚啉基（CDNI）γ-氨基丁酸（GABA）衍生物。CDNI-GABA 的双光子激发可快速激活脑切片神经元中的 GABA 能电流，轴向分辨率约为 2 微米，并实现 GABA-A 受体的高分辨率功能映射。GABA（主要的抑制性神经递质）的双光子光解，应该可以允许对受体功能和突触整合进行详细研究，达到亚细胞精度。
