双色双光子谷氨酸和 GABA 的光解笼。
Two-color, two-photon uncaging of glutamate and GABA.
机构信息
Department of Pharmacology and Physiology, Drexel University College of Medicine, Philadelphia, Pennsylvania, USA.
出版信息
Nat Methods. 2010 Feb;7(2):123-5. doi: 10.1038/nmeth.1413. Epub 2009 Dec 27.
Abstract
We developed a caged GABA (gamma-aminobutyric acid), which, when combined with an appropriate caged glutamate, allows bimodal control of neuronal membrane potential with subcellular resolution using optically independent two-photon uncaging of each neurotransmitter. We used two-color, two-photon uncaging to fire and block action potentials from rat hippocampal CA1 neurons in brain slices with 720-nm and 830-nm light, respectively. Our method should be generalizable to other chemical messenger pairs.
摘要
我们开发了一种笼形 GABA（γ-氨基丁酸），与适当的笼形谷氨酸结合使用，可以通过对两种神经递质进行光独立的双光子光解，实现亚细胞分辨率的神经元膜电位的双模控制。我们使用双色、双光子光解，分别用 720nm 和 830nm 的光从脑片的大鼠海马 CA1 神经元中引发和阻断动作电位。我们的方法应该可以推广到其他化学信使对。
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