Research Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China.
Analyst. 2011 May 7;136(9):1892-7. doi: 10.1039/c1an15014f. Epub 2011 Mar 4.
A strategy for the determination of the presence of thiol-containing amino acids was successfully established by simply assembling copper chloride and xylenol orange (3,3'-bis[N,N-bis(carboxymethyl)aminomethyl]-o-cresolsulfonephthalein trisodium salt; XO) in a 1 : 1 molar ratio in quasi-physiological water solution (pH 6.0). The copper(II)-XO ensemble was highly selective for thiol species such as cysteine, homocysteine, and glutathione without interference from other amino acids and could quantitatively detect thiol in the range from 10 to 200 μM with a linear relationship having an average molar absorbance constant of 6530 L mol(-1) cm(-1) in pure water. The whole recognition process for thiol gave rise to a rapid visual color change from purple-red to yellow which can be observed simultaneously with the naked-eye.
成功建立了一种简单地将氯化铜和二甲酚橙（3,3'-双[N,N-双（羧甲基）氨甲基]-邻-甲酚磺酰基邻苯二酚三钠盐；XO）以 1:1 摩尔比组装在近生理水溶剂（pH 6.0）中以确定含硫氨基酸存在的策略。铜（II）-XO 配合物对巯基物质如半胱氨酸、高半胱氨酸和谷胱甘肽具有高度选择性，不受其他氨基酸的干扰，并且可以在 10 至 200 μM 的范围内定量检测巯基，在纯水中的平均摩尔吸光常数为 6530 L mol(-1) cm(-1)。巯基的整个识别过程导致从紫红色到黄色的快速视觉颜色变化，肉眼可以同时观察到。
