Jia Hongmin, Yang Ming, Meng Qingtao, He Guangjie, Wang Yue, Hu Zhizhi, Zhang Run, Zhang Zhiqiang
Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan 114051, China.
Department of Forensic Medicine, Xinxiang Medical University, Xinxiang, He'nan 453003, China.
Sensors (Basel). 2016 Jan 11;16(1):79. doi: 10.3390/s16010079.
A fluorescence chemosensor, 2-hydroxy-1-naphthaldehyde azine (HNA) was designed and synthesized for sequential detection of Cu(2+) and biothiols. It was found that HNA can specifically bind to Cu(2+) with 1:1 stoichiometry, accompanied with a dramatic fluorescence quenching and a remarkable bathochromic-shift of the absorbance peak in HEPES buffer. The generated HNA-Cu(2+) ensemble displayed a "turn-on" fluorescent response specific for biothiols (Hcy, Cys and GSH) based on the displacement approach, giving a remarkable recovery of fluorescence and UV-Vis spectra. The detection limits of HNA-Cu(2+) to Hcy, Cys and GSH were estimated to be 1.5 μM, 1.0 μM and 0.8 μM, respectively, suggesting that HNA-Cu(2+) is sensitive enough for the determination of thiols in biological systems. The biocompatibility of HNA towards A549 human lung carcinoma cell, was evaluated by an MTT assay. The capability of HNA-Cu(2+) to detect biothiols in live A549 cells was then demonstrated by a microscopy fluorescence imaging assay.
设计并合成了一种荧光化学传感器2-羟基-1-萘甲醛嗪（HNA），用于顺序检测Cu(2+)和生物硫醇。研究发现，HNA能以1:1的化学计量比与Cu(2+)特异性结合，同时在HEPES缓冲液中伴随显著的荧光猝灭和吸收峰的明显红移。基于置换法，生成的HNA-Cu(2+)组合对生物硫醇（同型半胱氨酸、半胱氨酸和谷胱甘肽）表现出“开启”荧光响应，荧光和紫外-可见光谱有显著恢复。HNA-Cu(2+)对同型半胱氨酸、半胱氨酸和谷胱甘肽的检测限分别估计为1.5 μM、1.0 μM和0.8 μM，表明HNA-Cu(2+)对生物体系中硫醇的测定足够灵敏。通过MTT法评估了HNA对A549人肺癌细胞的生物相容性。然后通过显微镜荧光成像分析证明了HNA-Cu(2+)在活A549细胞中检测生物硫醇的能力。
