Department of Medical Biosciences/Pathology, Umeå University, Sweden.
Acta Derm Venereol. 2011 Jun;91(4):392-7. doi: 10.2340/00015555-1086.
Psoriasis is an inflammatory skin disease in which dysregulation of p63, a member of the p53 family that is crucial for skin development and maintenance, has been demonstrated. Involvement of miR-203, miR-21 and miR-125b, small non-coding RNAs implicated in the regulation of p63 or p53, has been suggested in the patho-genesis of psoriasis. To elucidate the roles of p63 and p63-related microRNAs in psoriasis and to increase our understanding of the mechanisms of narrow-band ultraviolet B (NB-UVB) phototherapy, we studied the effects of NB-UVB treatment on the expression of these molecules. Skin biopsies from 12 psoriasis patients were collected before, during and after NB-UVB therapy. Real-time PCR and immunohistochemistry showed that p63 expression was not significantly affected, whereas NB-UVB phototherapy significantly decreased expression of miR-21 (p = 0.003) and increased miR-125b levels (p = 0.003). The results indicate that the unresolved p63 abnormality in treated epidermis may play a role in maintenance of this disease.
银屑病是一种炎症性皮肤病，已经证明其 p63 失调，p63 是 p53 家族的成员，对皮肤的发育和维持至关重要。miR-203、miR-21 和 miR-125b 参与 p63 或 p53 的调节，已被提示参与银屑病的发病机制。为了阐明 p63 和 p63 相关 microRNAs 在银屑病中的作用，并增加我们对窄带紫外线 B（NB-UVB）光疗机制的理解，我们研究了 NB-UVB 治疗对这些分子表达的影响。从 12 名银屑病患者的皮肤活检中采集了治疗前、治疗中和治疗后的标本。实时 PCR 和免疫组织化学显示，p63 的表达没有明显影响，而 NB-UVB 光疗显著降低了 miR-21 的表达（p = 0.003），并增加了 miR-125b 的水平（p = 0.003）。结果表明，治疗表皮中未解决的 p63 异常可能在维持这种疾病中发挥作用。
