Prince Henry's Institute of Medical Research, PO Box 5152, Clayton, VIC, 3168, Australia.
J Mol Histol. 2011 Apr;42(2):161-6. doi: 10.1007/s10735-011-9319-5. Epub 2011 Mar 4.
The uterus undergoes dramatic remodelling in preparation for embryo implantation and pregnancy establishment. A receptive uterus is pivotal for embryo attachment, implantation and the eventual formation of a hemochorial placenta. We have previously identified by proteomics that tropomyosin alpha-4 chain (TPM4), protein disulfide isomerase A1 (PDIA1) and src substrate cortactin 8 (SRC8) were up regulated in the decidualized stromal cells during the late secretory phase of the menstrual cycle in women. These three proteins are associated with cytoskeletal remodelling. This study determined the localization of these three cytoskeletal proteins in the fetal-maternal interface including the decidual cells in the 1st trimester of pregnancy in women and rhesus monkeys. Immunohistochemical analysis revealed that TPM4, PDIA1 and SRC8 were all expressed by the decidual cells and the wall of the spiral arterioles in pregnant women. Similar expression pattern were also found in the rhesus monkey. In addition, TPM4, PDIA and SRC8 were also localized to the trophoblast cells, further highlighting the importance of these cytoskeletal remodelling proteins in early pregnancy.
子宫在为胚胎植入和妊娠建立做准备时会经历剧烈的重塑。一个有接受能力的子宫对于胚胎附着、植入和最终形成绒毛膜胎盘至关重要。我们之前通过蛋白质组学鉴定到，在女性月经周期的分泌晚期，蜕膜化基质细胞中，原肌球蛋白 α-4 链（TPM4）、蛋白二硫键异构酶 A1（PDIA1）和Src 底物连接蛋白 8（SRC8）上调。这三种蛋白质与细胞骨架重塑有关。本研究确定了这三种细胞骨架蛋白在胎儿-母体界面的定位，包括女性和食蟹猴妊娠早期蜕膜细胞和螺旋动脉壁中的定位。免疫组织化学分析显示，TPM4、PDIA1 和 SRC8 均在孕妇的蜕膜细胞和螺旋动脉壁中表达。在食蟹猴中也发现了类似的表达模式。此外，TPM4、PDIA 和 SRC8 也定位于滋养层细胞，进一步强调了这些细胞骨架重塑蛋白在早期妊娠中的重要性。
