Dimitriadis E, Robb L, Liu Y-X, Enders A C, Martin H, Stoikos C, Wallace E, Salamonsen L A
Prince Henry's Institute of Medical Research, PO Box 5152, Clayton, Victoria 3168, Australia.
Reprod Biol Endocrinol. 2003 Apr 11;1:34. doi: 10.1186/1477-7827-1-34.
Embryo implantation, endometrial stromal cell decidualization and formation of a functional placenta are critical processes in the establishment and maintenance of pregnancy. Interleukin (IL)-11 signalling is essential for adequate decidualization in the mouse uterus and IL-11 promotes decidualization in the human. IL-11 action is mediated via binding to the specific IL-11 receptor alpha (IL-11Ralpha). The present study examined immunoreactive IL-11 and IL-11Ralpha in cycling rhesus monkey endometrium, at implantation sites in cynomolgus and rhesus monkeys and in human first trimester decidua and defined distinct spatial and temporal patterns. In cycling rhesus monkey endometrium, IL-11 and IL-11Ralpha increased in both basalis and functionalis regions during the secretory compared with the proliferative phase, with changing cellular locations in luminal and glandular epithelium and stroma. The patterns were similar overall to those previously described in human endometrium. Differences were seen in immunostaining during implantation in cynomologus and rhesus monkey. In the cynomolgus, very little staining for IL-11 or IL-11Ralpha was seen in syncytio- and cyto-trophoblast cells in the villi between days 12 and 150 of pregnancy although there was moderate staining in cytotrophoblast in the shell between days 12 and 17 and in subpopulations of cytotrophoblast cells invading the arteries at day 17. By contrast in the rhesus monkey between days 24 and 35 of pregnancy and in human first trimester placenta, cyto- and syncytio-trophoblast in the villi but not cytotrophoblast in the shell were positively stained. The most intense staining for both IL-11 and IL-11Ralpha was present within the decidua in the maternal component of implantation sites in all three primates but moderate staining was also present in maternal vascular smooth muscle and glands perivascular cells and epithelial plaques. These results are consistent with a role for IL-11 both during decidualization and placentation in primates.
胚胎着床、子宫内膜基质细胞蜕膜化以及功能性胎盘的形成是妊娠建立和维持过程中的关键环节。白细胞介素（IL）-11信号通路对于小鼠子宫充分的蜕膜化至关重要，并且IL-11可促进人类的蜕膜化。IL-11通过与特异性白细胞介素-11受体α（IL-11Rα）结合来介导其作用。本研究检测了恒河猴周期性子宫内膜、食蟹猴和恒河猴着床部位以及人类孕早期蜕膜中免疫反应性IL-11和IL-11Rα，并确定了不同的时空模式。在恒河猴周期性子宫内膜中，与增殖期相比，分泌期基底区和功能区的IL-11和IL-11Rα均增加，其在腔上皮、腺上皮和基质中的细胞定位也发生变化。总体模式与先前在人类子宫内膜中描述的相似。在食蟹猴和恒河猴着床期间的免疫染色存在差异。在食蟹猴中，妊娠12至150天期间绒毛中的合体滋养层细胞和细胞滋养层细胞中几乎未见IL-11或IL-11Rα染色，尽管在妊娠12至17天期间壳中的细胞滋养层有中度染色，且在第17天侵入动脉的细胞滋养层细胞亚群中有染色。相比之下，在恒河猴妊娠24至35天期间以及人类孕早期胎盘中，绒毛中的细胞滋养层和合体滋养层呈阳性染色，而壳中的细胞滋养层未染色。在所有三种灵长类动物的着床部位母体成分的蜕膜中，IL-11和IL-11Rα的染色最为强烈，但母体血管平滑肌、血管周围腺细胞和上皮斑中也有中度染色。这些结果与IL-11在灵长类动物蜕膜化和胎盘形成过程中的作用一致。
Zotero 插件
