Harland J, Brown S M
Institute of Neurological Sciences, Southern General Hospital, Neurovirology Research Laboratories, University of Glasgow, Scotland, UK.
Methods Mol Med. 1998;10:1-8. doi: 10.1385/0-89603-347-3:1.
Whether herpes simplex virus (HSV) is viewed as a pathogen or as a model eukaryotic system, it is virtually certain that any experimental work will require the virus to be grown and assayed. The following chapter is therefore seen as the fundamental first step before embarking on more intellectually and technically challenging technology. Its importance should not however be underestimated. It never fails to surprize us that people who describe themselves as virologists have little understanding of the basic requirements needed to attain a contamination-free, high-titer, low particle:plaque-forming units (PFU) ratio, genetically pure virus stock.
无论单纯疱疹病毒（HSV）被视为病原体还是真核生物模型系统，几乎可以肯定的是，任何实验工作都需要培养和检测该病毒。因此，以下章节被视为在开展更具智力和技术挑战性的技术之前的基本第一步。然而，其重要性不应被低估。我们总是惊讶地发现，那些自称病毒学家的人对获得无污染、高滴度、低颗粒：空斑形成单位（PFU）比率、基因纯的病毒储备所需的基本要求知之甚少。
