Latchman D S, Kemp L M
Medical Molecular Biology Unit, University College and Middlesex School of Medicine, London, UK.
Methods Mol Biol. 1992;8:191-200. doi: 10.1385/0-89603-191-8:191.
In order to use herpes simplex virus (HSV) as a vector for the transmission and expression of foreign genes, it is obviously necessary to be able to prepare stocks of the virus and to propagate both HSV and the recombinant viruses derived from it. Similarly, the introduction of foreign genes into the virus will require the purification of viral DNA for use as a cloning vector, and preparation and analysis of viral DNA will also be necessary in order to characterize the recombinant viruses produced during the cloning procedures. This chapter will therefore discuss in turn the procedures used in our laboratory for the growth of HSV, and for the preparation of viral DNA.
为了将单纯疱疹病毒（HSV）用作外源基因的传递和表达载体，显然必须能够制备病毒毒株，并繁殖HSV及其衍生的重组病毒。同样，将外源基因导入病毒需要纯化病毒DNA用作克隆载体，并且为了鉴定克隆过程中产生的重组病毒，也需要制备和分析病毒DNA。因此，本章将依次讨论我们实验室中用于HSV培养以及病毒DNA制备的方法。
