Niepel Marcus S, Peschel Dieter, Groth Thomas
Biomedical Materials Group, Department of Pharmaceutical Technology and Biopharmacy, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Halle, Saale, Germany.
Int J Artif Organs. 2011 Feb;34(2):185-91. doi: 10.5301/ijao.2011.6395.
Tissue cells need to adhere to a biomaterial surface to promote their growth and differentiation and, thus, foster the integration of implants. As a result, surface features and their modification play an important role in biomedical applications. In this study, the layer-by-layer (LbL) technique was used to design self-assembled polyelectrolyte multilayer (PEM) coatings of polyethyleneimine (PEI) and heparin (HEP) on glass, which will control the adhesion of primary human dermal fibroblasts in a model system. The study showed that, among other surface features, the wettability of surfaces can be controlled by changing the conditions during multilayer self-assembly. Here, the pH value of the HEP solution was adjusted to acidic or alkaline values for terminal layers, which also led to a change in multilayer growth. Further, the study revealed that plain terminal layers were rather cytophobic. Upon pre-adsorption of fibronectin (FN), a clear effect on cell adhesion and morphology in dependence on the pH setup was evident. Proliferation studies clearly showed that terminal layers, which impaired cell adhesion, also inhibited growth of human fibroblasts under serum-conditions. On the other hand, on layers with pronounced cell adhesion an elevated cell growth was also observed. As a result, HEP terminated multilayers are interesting for applications requiring cell repellent properties, whereas PEI terminated multilayers could be used to promote cell adhesion and growth on implant surfaces.
组织细胞需要附着在生物材料表面以促进其生长和分化，从而促进植入物的整合。因此，表面特征及其修饰在生物医学应用中起着重要作用。在本研究中，采用层层（LbL）技术在玻璃上设计了聚乙烯亚胺（PEI）和肝素（HEP）的自组装聚电解质多层（PEM）涂层，这将在模型系统中控制原代人皮肤成纤维细胞的粘附。研究表明，除其他表面特征外，通过改变多层自组装过程中的条件可以控制表面的润湿性。在此，将HEP溶液的pH值调节至酸性或碱性用于终端层，这也导致多层生长的变化。此外，研究表明普通终端层具有相当的细胞排斥性。在预吸附纤连蛋白（FN）后，对细胞粘附和形态依赖于pH设置有明显影响。增殖研究清楚地表明，损害细胞粘附的终端层在血清条件下也抑制人成纤维细胞的生长。另一方面，在具有明显细胞粘附的层上也观察到细胞生长增加。因此，HEP终止的多层膜对于需要细胞排斥特性的应用很有意义，而PEI终止的多层膜可用于促进植入物表面的细胞粘附和生长。
