Sørensen Lambert K
Section for Toxicology and Drug Analysis, Department of Forensic Medicine, Aarhus University, Brendstrupgaardsvej 100, Aarhus N, Denmark.
Biomed Chromatogr. 2012 Jan;26(1):1-5. doi: 10.1002/bmc.1615. Epub 2011 Mar 4.
Metformin is an anti-diabetic drug in the biguanide class which also includes phenformin and buformin. Because of the potential adverse effects of the biguanides, a reliable liquid chromatography-tandem mass spectrometry method using pneumatically assisted electrospray ionization was developed for the quantification of the drugs in both live and post-mortem human whole blood. The blood proteins were precipitated by the addition of a mixture of methanol and acetonitrile, and the extract was cleaned up by cation-exchange solid-phase extraction to eliminate ion suppression effects. The separation was performed by hydrophilic interaction liquid chromatography. Matrix-matched calibrants combined with isotope dilution of metformin were used for calibration. The detection limits were 0.01 mg/L for metformin and phenformin and the relative intra-laboratory reproducibility standard deviations were less than 6% at concentrations of 1-10 mg/L. The mean true recoveries were greater than 86%.
二甲双胍是双胍类抗糖尿病药物，该类还包括苯乙双胍和丁双胍。由于双胍类药物存在潜在不良反应，因此开发了一种可靠的液相色谱-串联质谱法，该方法采用气动辅助电喷雾电离技术，用于定量生前和死后人体全血中的这些药物。通过添加甲醇和乙腈的混合物使血液蛋白沉淀，提取物通过阳离子交换固相萃取进行净化，以消除离子抑制效应。采用亲水相互作用液相色谱进行分离。使用与二甲双胍同位素稀释相结合的基质匹配校准物进行校准。二甲双胍和苯乙双胍的检测限为0.01 mg/L，在1-10 mg/L浓度下，实验室内相对重现性标准偏差小于6%。平均真实回收率大于86%。
