Section for Toxicology and Drug Analysis, Department of Forensic Medicine, Aarhus University, Brendstrupgaardsvej 100, 8200 Aarhus N, Denmark.
J Anal Toxicol. 2012 Mar;36(2):116-22. doi: 10.1093/jat/bkr021.
A liquid chromatography-tandem mass spectrometry method, using pneumatically assisted electrospray ionization was developed for the determination of amiodarone, desethylamiodarone, propafenone, N-depropylpropafenone, 5-OH-propafenone, flecainide, and sotalol in human antemortem and postmortem whole blood. A mixture of methanol and acetonitrile was used to extract the samples, and the clear extracts obtained from the protein precipitation were injected directly onto an ethyl-linked phenyl LC column. The isotope dilution technique was applied for quantitative analysis. The relative intralaboratory reproducibility standard deviations were 5-9% at a concentration range of 1-5 mg/L and 9-12% at a concentration of 0.1 mg/L. The mean true recoveries were greater than 91% in the concentration range of 0.05-5 mg/L. The detection limits were in the range of 8-18 µg/L.
建立了一种采用气动辅助电喷雾电离的液相色谱-串联质谱法，用于检测人死后全血中的胺碘酮、去乙基胺碘酮、普罗帕酮、N-去丙基普罗帕酮、5-OH-普罗帕酮、氟卡尼和索他洛尔。甲醇和乙腈的混合物用于提取样品，从蛋白沉淀中得到的澄清提取物直接注入乙基键合苯基 LC 柱。同位素稀释技术用于定量分析。在浓度范围为 1-5 mg/L 时，相对实验室内重现性标准偏差为 5-9%，在浓度为 0.1 mg/L 时为 9-12%。在 0.05-5 mg/L 的浓度范围内，平均真实回收率大于 91%。检测限在 8-18 µg/L 范围内。
