Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin, People's Republic of China.
Luminescence. 2011 Nov-Dec;26(6):592-7. doi: 10.1002/bio.1276. Epub 2011 Mar 4.
A novel method was developed using capillary electrophoresis (CE) coupled with tris(2,2'-bipyridyl)ruthenium(II) electrogenerated chemiluminescence (ECL) for highly sensitive detection of metformin hydrochloride (MH) derivatizatized with acetaldehyde. The precolumn derivatization of MH with acetaldehyde was performed in phosphate buffer solution (0.3 mol/L, pH 7.5) at room temperature for 120 min. The effects of acetaldehyde concentration, buffer pH, electrokinetic voltage and injection time were investigated. Under optimized detection conditions, the MH ECL detection sensitivity was more than 120 times that without derivatization. The linear concentration range for MH was 0.001-15.00 μg/mL (with a correlation coefficient of 0.9992). The detection limit was 0.31 ng/mL with a signal:noise ratio of 3. The recoveries of MH in human urine were in the range 98.50-99.72%. Copyright © 2011 John Wiley & Sons, Ltd.
开发了一种新的方法，使用毛细管电泳（CE）与三（2,2'-联吡啶）钌（II）电生化学发光（ECL）结合，用于高度灵敏地检测盐酸二甲双胍（MH）与乙醛衍生化。在室温下，在磷酸盐缓冲溶液（0.3 mol/L，pH 7.5）中，将 MH 与乙醛进行预柱衍生化 120 分钟。考察了乙醛浓度、缓冲液 pH 值、电动电压和进样时间的影响。在优化的检测条件下，MH 的 ECL 检测灵敏度比未衍生化时提高了 120 多倍。MH 的线性浓度范围为 0.001-15.00 μg/mL（相关系数为 0.9992）。检测限为 0.31 ng/mL，信噪比为 3。MH 在人尿中的回收率在 98.50-99.72%范围内。版权所有©2011 年 John Wiley & Sons, Ltd.
