State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310003, People's Republic of China.
Luminescence. 2012 Jan-Feb;27(1):63-8. doi: 10.1002/bio.1326. Epub 2011 Jul 11.
A rapid, sensitive and simple electrochemiluminescence method for the determination of 5-hydroxytryptamine (5-HT) using capillary electrophoresis was proposed. The experimental parameters, including the detection potential, the concentration of Ru(bpy)(3)(2+), the concentration and pH of phosphate buffer for separation and detection, the injection voltage and time and the separation voltage on the determination of 5-HT, were optimized. Under the optimized conditions, the linear concentration range for 5-HT was 3.5 × 10(-9) -5.1 × 10(-3) mol/L, with a detection limit of 5 × 10(-10) mol/L. The relative standard deviations (RSDs) of the ECL intensity and the migration times for six continuous injections of 1.0 µmol/L 5-HT were 2.48% and 1.3%, respectively. The method was successfully applied to 5-HT assay in samples of human serum in 5 min and the extraction recoveries with spiked serum samples were over 94.4%.
提出了一种快速、灵敏、简单的电化学发光法，用于毛细管电泳测定 5-羟色胺（5-HT）。优化了实验参数，包括检测电位、Ru（bpy）（3）（2+）浓度、分离和检测用磷酸盐缓冲液的浓度和 pH 值、进样电压和时间以及分离电压对 5-HT 测定的影响。在优化条件下，5-HT 的线性浓度范围为 3.5×10（-9）-5.1×10（-3）mol/L，检测限为 5×10（-10）mol/L。连续注入 6 次 1.0µmol/L 5-HT 时，ECL 强度和迁移时间的相对标准偏差（RSD）分别为 2.48%和 1.3%。该方法成功应用于 5 分钟内人血清中 5-HT 的测定，加标血清样品的提取回收率超过 94.4%。
