Han Jian, Ye Lin, Zhang Aiying, Feng Zengguo
School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2010 Dec;27(6):1274-9.
A novel diisocyanate, i. e. lysine ethyl ester diisocyanate (LDI), was prepared by the present authors. Poly (epsilon-caprolactone) (PCL) (M(n) = 2000) was used for reacting with LDI to form prepolymer, and then the chain was extended with butanediol (BDO) to form polyurethane (PU). PU was characterized by gel permeation chromatography, FTIR and 1H-NMR. Mechanical properties test revealed that PU possesses excellent tensile strength. Hydrolytic degradation and enzymatic degradation of PU films showed that PU is biodegradable. Finally, vascular scaffold of PU was fabricated by electrospinning. Morphological and biomechanical properties of scaffold were examined. The tensile strength was 8MPa, suture retention strength 12N, porosity 75% and burst pressure strength 150-170 kPa. Cytotoxicity and cell adhesion showed that PU scaffolds are biocompatible. These results demonstrate that PU vascular scaffolds possess excellent physical strength and biocompatibility and can be developed as substitutes for native blood vessels.
本文作者制备了一种新型二异氰酸酯，即赖氨酸乙酯二异氰酸酯（LDI）。使用聚（ε-己内酯）（PCL）（数均分子量M(n)=2000）与LDI反应形成预聚物，然后用丁二醇（BDO）进行扩链以形成聚氨酯（PU）。通过凝胶渗透色谱法、傅里叶变换红外光谱法和核磁共振氢谱对PU进行了表征。力学性能测试表明，PU具有优异的拉伸强度。PU膜的水解降解和酶解降解表明PU是可生物降解的。最后，通过静电纺丝制备了PU血管支架。对支架的形态和生物力学性能进行了检测。其拉伸强度为8MPa，缝线保留强度为12N，孔隙率为75%，爆破压力强度为150 - 170kPa。细胞毒性和细胞黏附性表明PU支架具有生物相容性。这些结果表明，PU血管支架具有优异的物理强度和生物相容性，可作为天然血管的替代品进行开发。
