Hayashi Y, Sugita K, Nakazawa S, Abe T, Kojima S, Inaba T, Hanada R, Yamamoto K
Division of Hematology/Oncology, Saitama Children's Medical Center, Japan.
Leukemia. 1990 Feb;4(2):121-6.
We performed cytogenetic and immunologic studies of blast cells from 13 children with acute mixed lineage leukemia (AMLL) to discern patterns of chromosome alteration and antigen expression that would assist in classification of this disease entity. Six patients with 11q23 translocations--including four with the t(11;19), one with the t(9;11), and one with the t(1;11)--were characterized by a young age and hyperleukocytosis. A B cell-associated antigen (CD19) and HLA-DR antigens were expressed by blast cells from all patients; only one case was positive for the common acute lymphocytic leukemia antigen (CALLA, CD10). A myeloid-associated antigen (CD13) was expressed by blast cells from one patient at diagnosis and from another at relapse; it was also expressed by cells from the remaining four patients after brief in vitro culture without addition of differentiating agents. Four patients with t(9;22)(q34;q11) were characterized by an older age and hyperleukocytosis. Each of these cases was positive for CD13, CD19, and HLA-DR, and three were positive for CALLA. The 11q23 translocation was associated with CALLA- ALL marked by a myeloid phenotype, whereas the t(9;22) occurred in cases of acute myeloid leukemia with a CALLA+ lymphoid phenotype. One case had a 7q35-q36 translocation, which involves the region of the T cell receptor beta-chain gene. Our results suggest that karyotypic alterations can be used to refine the classification of AMLL.
我们对13例急性混合谱系白血病（AMLL）患儿的原始细胞进行了细胞遗传学和免疫学研究，以识别有助于该疾病实体分类的染色体改变模式和抗原表达情况。6例发生11q23易位的患者——包括4例t(11;19)、1例t(9;11)和1例t(1;11)——具有年龄小和白细胞增多的特点。所有患者的原始细胞均表达一种B细胞相关抗原（CD19）和HLA-DR抗原；只有1例普通急性淋巴细胞白血病抗原（CALLA，CD10）呈阳性。1例患者诊断时及另1例复发时的原始细胞表达一种髓系相关抗原（CD13）；在未添加分化剂的情况下进行短暂体外培养后，其余4例患者的细胞也表达该抗原。4例t(9;22)(q34;q11)患者的特点是年龄较大和白细胞增多。这些病例中的每一例CD13、CD19和HLA-DR均呈阳性，3例CALLA呈阳性。11q23易位与以髓系表型为特征的CALLA-ALL相关，而t(9;22)发生于具有CALLA+淋巴表型的急性髓系白血病病例中。1例发生7q35-q36易位，该易位涉及T细胞受体β链基因区域。我们的结果表明，核型改变可用于完善AMLL的分类。
