Sox2 和 Oct4 在预测肝癌患者肝切除术后生存中的作用。
Role of Sox2 and Oct4 in predicting survival of hepatocellular carcinoma patients after hepatectomy.
机构信息
Department of Hepatobiliary Oncology, Sun Yat-sen University Cancer Center, Guangzhou, GD 510060, China.
出版信息
Clin Biochem. 2011 Jun;44(8-9):582-9. doi: 10.1016/j.clinbiochem.2011.02.012. Epub 2011 Mar 1.
OBJECTIVES
The present study was aimed to explore the prognostic strength of Sox2 and Oct4A in hepatocellular carcinoma (HCC).
DESIGN AND METHODS
We investigated the expression of Sox2 and Oct4A in five hepatoma cell lines, one immortalized normal liver cell line, HCC tissues with matched nontumorous liver tissues and normal liver tissues by reverse transcription-polymerase chain reaction.
RESULTS
Sox2 and Oct4A mRNA were overexpressed in hepatoma cell lines and tumor tissues. Sox2 or Oct4A positive expression was significantly associated with an aggressive phenotype. Both univariate and multivariate analyses revealed that Sox2 or Oct4A was an independent prognostic factor for HCC. When using subgroup analysis, the patients with a co-expression of Sox2/Oct4A had the poorest prognosis. Further analysis demonstrated that Sox2 alone or Sox2/Oct4A could stratify outcome in HCC patients with early stage.
CONCLUSIONS
Sox2 and Oct4A can be novel predictors of poor prognosis for patients undergoing resection of HCC.
目的
本研究旨在探讨 Sox2 和 Oct4A 在肝细胞癌（HCC）中的预后价值。
设计与方法
通过逆转录-聚合酶链反应，我们检测了 Sox2 和 Oct4A 在五株肝癌细胞系、一株永生化正常肝细胞系、HCC 组织及其配对的非肿瘤肝组织和正常肝组织中的表达。
结果
Sox2 和 Oct4A mRNA 在肝癌细胞系和肿瘤组织中过度表达。Sox2 或 Oct4A 的阳性表达与侵袭性表型显著相关。单因素和多因素分析均表明 Sox2 或 Oct4A 是 HCC 的独立预后因素。亚组分析显示，同时表达 Sox2/Oct4A 的患者预后最差。进一步分析表明，Sox2 单独或 Sox2/Oct4A 可以对早期 HCC 患者的预后进行分层。
结论
Sox2 和 Oct4A 可作为 HCC 患者切除术后预后不良的新预测指标。
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