Imunology and Microbiology Lab, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Dent Mater. 2011 May;27(5):e81-6. doi: 10.1016/j.dental.2011.01.001. Epub 2011 Mar 3.
The aim of this study was to assess the cytotoxicity of orthodontic materials (brackets, wires, resin, elastomers and silver solder) using Saccharomyces cerevisiae as a model organism. The induction of cytotoxicity was assessed by two different tests using the wild-type S. cerevisiae strain FF18733: (1) direct exposure to orthodontic materials in YPD broth, and (2) exposure to artificial commercial saliva pre-treated with orthodontic materials. Only the silver solder was tested in mutant S. cerevisiae strains to investigate the origin of the observed cytotoxicity. Colony forming units per mL counts were carried out in all experiments and compared to controls to detect significant survival differences. The results showed that only the silver solder induced significant cytotoxicity, which might have occurred via oxidative stress, although this mechanism is not completely understood. Moreover, S. cerevisiae proved to be a reliable and useful model microorganism for evaluating the cytotoxicity of clinical materials.
本研究旨在使用酿酒酵母作为模型生物来评估正畸材料（托槽、弓丝、树脂、弹性体和银焊料）的细胞毒性。通过使用野生型酿酒酵母菌株 FF18733 的两种不同测试来评估细胞毒性的诱导：（1）在 YPD 肉汤中直接暴露于正畸材料，以及（2）暴露于经正畸材料预处理的人工商业唾液。仅在突变型酿酒酵母菌株中测试银焊料，以研究观察到的细胞毒性的起源。在所有实验中都进行了每毫升形成菌落的单位计数，并与对照进行比较，以检测显著的生存差异。结果表明，只有银焊料引起了显著的细胞毒性，这可能是通过氧化应激引起的，尽管这种机制尚不完全清楚。此外，酿酒酵母被证明是评估临床材料细胞毒性的可靠和有用的模型微生物。
