Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel.
Virus Res. 2011 Jun;158(1-2):8-11. doi: 10.1016/j.virusres.2011.02.016. Epub 2011 Mar 3.
The capsid protein (CP) of Tomato yellow leaf curl virus-Israel (TYLCV-IL), encoded by the v1 gene, is the only known component of the viral capsid. Three point mutations introduced into the conserved NLS region of the CP were investigated. One mutant, in which the Arg at position 19 was converted to Leu, had the most significant effect on the CP-CP homotypic interaction as well as on CP's interaction with its nuclear receptor karyopherin α1 and with the protein GroEL. The latter has been suggested to protect the virions in the insect vector hemolymph. These effects were first observed by yeast two-hybrid assay and then confirmed in tobacco protoplasts by measuring fluorescence resonance energy transfer (FRET) between YFP- and CFP-tagged proteins. Most importantly, when the point mutation converting Arg 19 to Leu was introduced into the full-length TYLCV genome, it disrupted its ability to cause symptoms.
番茄黄曲叶病毒-以色列株（TYLCV-IL）的外壳蛋白（CP）由 v1 基因编码，是病毒衣壳的唯一已知组成部分。研究了引入 CP 保守核定位信号（NLS）区域的三个点突变。一个突变体将位置 19 的精氨酸突变为亮氨酸，对 CP-CP 同源相互作用以及 CP 与核受体载体蛋白α1 和 GroEL 蛋白的相互作用有最显著的影响。GroEL 被认为可以保护病毒粒子在昆虫载体血淋巴中。这些影响首先通过酵母双杂交实验观察到，然后通过测量 YFP 和 CFP 标记蛋白之间的荧光共振能量转移（FRET）在烟草原生质体中得到证实。最重要的是，当将 Arg 19 突变为 Leu 的点突变引入全长 TYLCV 基因组中时，它破坏了其引起症状的能力。
