直接光热技术快速定量测定酸樱桃品种中总花色苷含量。

Direct photothermal techniques for rapid quantification of total anthocyanin content in sour cherry cultivars.

机构信息

Department of Physics and Mathematics, University of West Hungary, Deak F. sq. 1, 9200 Mosonmagyaróvár, Hungary.

出版信息

Talanta. 2011 Apr 15;84(2):341-6. doi: 10.1016/j.talanta.2011.01.007. Epub 2011 Jan 15.

DOI:10.1016/j.talanta.2011.01.007

Abstract

The analytical performance of the newly proposed laser-based photoacoustic spectroscopy (PAS) and of optothermal window (OW) method for quantification of total anthocyanin concentration (TAC) in five sour cherry varieties is compared to that of the spectrophotometry (SP). High performance liquid chromatography (HPLC) was used to identify and quantify specific anthocyanins. Both, PAS and OW are direct methods that unlike SP and HPLC obviate the need for the extraction of analyte. The outcome of the study leads to the conclusion that PAS and OW are both suitable for quick screening of TAC in sour cherries. The correlation between the two methods and SP is linear with R(2)=0.9887 for PAS and R(2)=0.9918 for OW, respectively. Both methods are capable of the rapid determination of TAC in sour cherries without a need for a laborious sample pretreatment.

摘要

新提出的基于激光的光声光谱（PAS）和光热窗（OW）方法的分析性能，以及用于定量五种酸樱桃品种总花青素浓度（TAC）的分光光度法（SP）进行了比较。高效液相色谱法（HPLC）用于鉴定和定量特定的花青素。PAS 和 OW 都是直接方法，与 SP 和 HPLC 不同，它们不需要提取分析物。研究的结果得出结论，PAS 和 OW 都适合快速筛选酸樱桃中的 TAC。两种方法与 SP 的相关性均呈线性关系，PAS 的 R(2)=0.9887，OW 的 R(2)=0.9918。这两种方法都能够快速测定酸樱桃中的 TAC，而无需繁琐的样品预处理。