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新杏(Prunus armeniaca L.)品种中酚类化合物的表征与定量分析

Characterization and quantitation of phenolic compounds in new apricot (Prunus armeniaca L.) varieties.

作者信息

Ruiz David, Egea José, Gil María I, Tomás-Barberán Francisco A

机构信息

Research Group on Apricot Breeding, Department of Plant Breeding, and Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, Campus Universitario, Espinardo, Murcia, Spain.

出版信息

J Agric Food Chem. 2005 Nov 30;53(24):9544-52. doi: 10.1021/jf051539p.

Abstract

Thirty-seven apricot varieties, including four new releases (Rojo Pasión, Murciana, Selene, and Dorada) obtained from different crosses between apricot varieties and three traditional Spanish cultivars (Currot, Mauricio, and Búlida), were separated according to flesh color into four groups: white, yellow, light orange, and orange (mean hue angles in flesh were 88.1, 85.0, 77.6, and 72.4, respectively). Four phenolic compound groups, procyanidins, hydroxycinnamic acid derivatives, flavonols, and anthocyanins, were identified by HPLC-MS/MS and individually quantified using HPLC-DAD. Chlorogenic and neochlorogenic acids, procyanidins B1, B2, and B4, and some procyanidin trimers, quercetin 3-rutinoside, kaempferol 3-rhamnosyl-hexoside and quercetin 3-acetyl-hexoside, cyanidin 3-rutinoside, and 3-glucoside, were detected and quantified in the skin and flesh of the different cultivars. The total phenolics content, quantified as the addition of the individual compounds quantified by HPLC, ranged between 32.6 and 160.0 mg 100 g(-1) of edible tissue. No correlation between the flesh color and the phenolic content of the different cultivars was observed.

摘要

37个杏品种,包括通过杏品种间不同杂交获得的4个新品种(红激情、穆尔西亚纳、塞勒涅和多拉达)以及3个西班牙传统品种(库罗特、毛里西奥和布利达),根据果肉颜色分为四组:白色、黄色、浅橙色和橙色(果肉的平均色调角分别为88.1、85.0、77.6和72.4)。通过HPLC-MS/MS鉴定了4种酚类化合物组,即原花青素、羟基肉桂酸衍生物、黄酮醇和花青素,并使用HPLC-DAD对其进行单独定量。在不同品种的果皮和果肉中检测并定量了绿原酸和新绿原酸、原花青素B1、B2和B4以及一些原花青素三聚体、槲皮素3-芸香糖苷、山奈酚3-鼠李糖基己糖苷和槲皮素3-乙酰己糖苷、矢车菊素3-芸香糖苷和3-葡萄糖苷。以HPLC定量的各化合物相加来定量的总酚含量在可食组织的32.6至160.0 mg 100 g(-1)之间。未观察到不同品种的果肉颜色与酚类含量之间存在相关性。

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