Zhou Zhi-Jun, Zhang Yan-Xia, Chang Yan-Lin, Yang Ming-Ru
Hebei University, Baoding, China.
Yi Chuan. 2011 Jan;33(1):75-80. doi: 10.3724/sp.j.1005.2011.00075.
The mitochondrial cytochrome oxidase I (mtDNA-COI) gene was used to investigate the intraspecific genetic subdivision among 12 geographical populations of Gampsocleis sedakovii distributed in Jilin, Liaoning, Inner Mongolia, Hebei, and Sichuan provinces, China. As for the 626 bp mtDNA-COI sequences of 36 individuals, 29 haplotypes and 71 (11.3%) polymorphic sites were detected, including 37 parsimony informative and 34 singleton variable sites. The results of the analysis of molecular variance (AMOVA) showed that the percentage of variation within populations (37.23%) was less than that among populations. The Fst value was 0.62770 (P< 0.001), and the genetic divergence among populations was significant. Maximum parsimony (MP) analysis revealed two clearly differentiated branch (Ⅰ and Ⅱ) with high bootstrap support (100%). However, these two branches did not correspond in the subspecies distinction based on morphological character. Based on the habitats analysis, we preliminary inferred that morphological divergence between Gampsocleis se-dakovii sedakovii and Gampsocleis sedakovii obscura was the result of habitat dissimilarity. The majority of the G. se-dakovii individuals collected in NTL (Tongliao, Inner Mongolia) were identified as the members of branch Ⅱ, but one individual (NTL-3) belonged to branch Ⅰ. Thus, at least this locality was occupied by two phylogenetically independent individuals. Haplotypes (H10) were shared by three localities, NTL, NEWK (Ewenke, Inner Mongolia) and JJL (Jilin, Jilin), indicated that they shared a common ancestor. We approved that Northeastern China (Manchuria China) was the center of G. sedakovii genetic differentiation, but disapproved the subspecies distinction based on morphological character.
利用线粒体细胞色素氧化酶I（mtDNA-COI）基因，对分布于中国吉林、辽宁、内蒙古、河北和四川等省份的12个地理种群的桑螽进行种内遗传分化研究。对36个个体的626 bp mtDNA-COI序列分析发现，共检测到29个单倍型和71个（11.3%）多态性位点，其中包括37个简约信息位点和34个单可变位点。分子方差分析（AMOVA）结果表明，种群内变异百分比（37.23%）小于种群间变异百分比。Fst值为0.62770（P<0.001），种群间遗传分化显著。最大简约法（MP）分析显示，有两个明显分化的分支（Ⅰ和Ⅱ），自展支持率较高（100%）。然而，这两个分支在基于形态特征的亚种划分上并不对应。基于栖息地分析，我们初步推断，桑螽指名亚种和暗桑螽之间的形态差异是栖息地差异的结果。在内蒙古通辽采集的大多数桑螽个体被鉴定为分支Ⅱ的成员，但有一个个体（NTL-3）属于分支Ⅰ。因此，至少在这个地点存在两个系统发育上独立的个体。单倍型（H10）在内蒙古通辽、内蒙古鄂温克和吉林吉林这三个地点共享，表明它们有共同的祖先。我们认可中国东北地区（满洲地区）是桑螽遗传分化的中心，但不认可基于形态特征的亚种划分。
