Zhong Qiang, Zhao Shu-Hong
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Yi Chuan. 2011 Feb;33(2):123-30. doi: 10.3724/sp.j.1005.2011.00123.
Due to specific recognization of DNA sequences and designability, zinc finger nucleases (ZFN) has been used in knock in and knock out genes. Because of high ratio of homologous recombination of DSB-GT induces by ZFN, ZFN technology has been considered as the most powerful tool for gene modification. It has been successfully used at cell or embryo levels in plants and animals. Under the rapid development of high affinity zinc finger protein (ZFP), this technique will be applied to genetic engineering and breeding extensively in the future. This review discussed the DNA recognization mechanism and double strand break gene targeting (DSB-GT) of zinc finger nucleases (ZFN). Some application examples of ZFN were summarized.
由于锌指核酸酶（ZFN）对DNA序列具有特异性识别和可设计性，已被用于基因敲入和敲除。由于ZFN诱导的双链断裂引导的基因靶向（DSB-GT）具有较高的同源重组率，ZFN技术被认为是基因修饰最强大的工具。它已在植物和动物的细胞或胚胎水平上成功应用。在高亲和力锌指蛋白（ZFP）快速发展的情况下，该技术未来将在基因工程和育种中得到广泛应用。本文综述了锌指核酸酶（ZFN）的DNA识别机制和双链断裂基因靶向（DSB-GT），并总结了ZFN的一些应用实例。
