Benchimol M, Batista C, De Souza W
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.
J Submicrosc Cytol Pathol. 1990 Jan;22(1):39-45.
The pathogenic protozoan Tritrichomonas foetus is able to ingest polystyrene particles with a diameter up to 1.0 micron. Trichomonas vaginalis, however, ingest particles as large as 4.4 microns in diameter. The particles are found within cytoplasmic membrane-bounded vacuoles. Morphometrical analysis showed that T. vaginalis presents a higher endocytic activity than T. foetus. Coating of the polystyrene particles with cationized ferritin increases their binding to the parasite surface but does not interfere with their ingestion. In contrast, coating with laminin significantly increased the uptake of the particles by both parasites while coating with fibronectin potentiates the ingestion of the particles only by T. foetus. These observations suggest the presence of laminin- and fibronectin-binding sites on the surface of trichomonads, an observation which is in agreement with the recent description of a receptor for laminin on the surface of trichomonads.
致病性原生动物胎儿三毛滴虫能够摄取直径达1.0微米的聚苯乙烯颗粒。然而，阴道毛滴虫能摄取直径达4.4微米的颗粒。这些颗粒存在于细胞质膜包被的液泡内。形态计量学分析表明，阴道毛滴虫的内吞活性高于胎儿三毛滴虫。用阳离子铁蛋白包被聚苯乙烯颗粒可增加其与寄生虫表面的结合，但不干扰其摄取。相反，用层粘连蛋白包被可显著增加两种寄生虫对颗粒的摄取，而用纤连蛋白包被仅增强胎儿三毛滴虫对颗粒的摄取。这些观察结果表明，滴虫表面存在层粘连蛋白和纤连蛋白结合位点，这一观察结果与最近关于滴虫表面层粘连蛋白受体的描述一致。
