Shine M B, Guruprasad K N, Anand Anjali
School of Life Sciences, Devi Ahilya University, Indore, India.
Bioelectromagnetics. 2011 Sep;32(6):474-84. doi: 10.1002/bem.20656. Epub 2011 Mar 4.
Experiments were conducted to study the effect of static magnetic fields on the seeds of soybean (Glycine max (L.) Merr. var: JS-335) by exposing the seeds to different magnetic field strengths from 0 to 300 mT in steps of 50 mT for 30, 60, and 90 min. Treatment with magnetic fields improved germination-related parameters like water uptake, speed of germination, seedling length, fresh weight, dry weight and vigor indices of soybean seeds under laboratory conditions. Improvement over untreated control was 5-42% for speed of germination, 4-73% for seedling length, 9-53% for fresh weight, 5-16% for dry weight, and 3-88% and 4-27% for vigor indices I and II, respectively. Treatment of 200 mT (60 min) and 150 mT (60 min), which were more effective than others in increasing most of the seedling parameters, were further explored for their effect on plant growth, leaf photosynthetic efficiency, and leaf protein content under field conditions. Among different growth parameters, leaf area, and leaf fresh weight showed maximum enhancement (more than twofold) in 1-month-old plants. Polyphasic chlorophyll a fluorescence (OJIP) transients from magnetically treated plants gave a higher fluorescence yield at the J-I-P phase. The total soluble protein map (SDS-polyacrylamide gel) of leaves showed increased intensities of the bands corresponding to a larger subunit (53 KDa) and smaller subunit (14 KDa) of Rubisco in the treated plants. We report here the beneficial effect of pre-sowing magnetic treatment for improving germination parameters and biomass accumulation in soybean.
进行了实验，通过将大豆（Glycine max (L.) Merr. var: JS-335）种子暴露于0至300 mT的不同磁场强度下，步长为50 mT，处理30、60和90分钟，来研究静磁场对大豆种子的影响。在实验室条件下，磁场处理改善了与发芽相关的参数，如大豆种子的吸水量、发芽速度、幼苗长度、鲜重、干重和活力指数。与未处理的对照相比，发芽速度提高了5 - 42%，幼苗长度提高了4 - 73%，鲜重提高了9 - 53%，干重提高了5 - 16%，活力指数I提高了3 - 88%，活力指数II提高了4 - 27%。进一步探究了200 mT（60分钟）和150 mT（60分钟）处理，这两种处理在增加大多数幼苗参数方面比其他处理更有效，研究了它们在田间条件下对植物生长、叶片光合效率和叶片蛋白质含量的影响。在不同的生长参数中，1月龄植株的叶面积和叶鲜重增幅最大（超过两倍）。经磁场处理的植株的多相叶绿素a荧光（OJIP）瞬变在J - I - P相具有更高的荧光产量。叶片的总可溶性蛋白质图谱（SDS - 聚丙烯酰胺凝胶）显示，处理植株中与Rubisco大亚基（53 KDa）和小亚基（14 KDa）相对应的条带强度增加。我们在此报告播种前磁处理对改善大豆发芽参数和生物量积累的有益作用。
