Waalkens-Berendsen D H, Koëter H B, Sinkeldam E J
TNO-CIVO Toxicology and Nutrition Institute, Zeist, The Netherlands.
Food Chem Toxicol. 1990 Jan;28(1):11-9. doi: 10.1016/0278-6915(90)90130-f.
The sugar replacer isomalt was fed to Wistar rats of both sexes throughout three successive generations at concentrations of 0, 2.5, 5 and 10% in the diet. A group of rats fed a diet containing 10% sucrose served as an additional control group. The initial mating was of 100 rats of each sex in each group. For subsequent matings 20 rats of each sex from each group were used. For each generation two litters were reared until they were at least 3 wk old. Feeding isomalt to rats for three successive generations did not induce any adverse effects on fertility, reproductive performance or development compared with control animals fed diets containing maize starch and sucrose instead of isomalt. The second litter of third-generation rats was given detailed gross and microscopic examinations 4 wk after weaning. A marked treatment-related change was an increase in the relative weight of the caecum (filled and empty), 4 wk after weaning in the second litter of third-generation rats fed 10% isomalt. There was also an increase in the relative weight of the filled caecum in males of the 5% isomalt group. These findings were not accompanied by diarrhoea or histological changes in the caecum. The results of the present study did not demonstrate any deleterious effects on the reproduction, maternal performance or development of rats fed isomalt at dietary levels of up to 10% over three successive generations.
在连续三代的时间里，将糖替代品异麦芽酮糖醇以0%、2.5%、5%和10%的浓度添加到Wistar大鼠的饮食中，雌雄大鼠均喂食该饮食。一组喂食含10%蔗糖饮食的大鼠作为额外的对照组。每组最初有100只雌雄大鼠用于交配。后续交配时，每组使用20只雌雄大鼠。每一代饲养两窝幼崽，直到它们至少3周龄。与喂食含玉米淀粉和蔗糖而非异麦芽酮糖醇饮食的对照动物相比，连续三代给大鼠喂食异麦芽酮糖醇对其生育力、生殖性能或发育未产生任何不良影响。对第三代大鼠的第二窝幼崽在断奶后4周进行详细的大体和显微镜检查。一个明显的与处理相关的变化是，在第三代大鼠第二窝中，喂食10%异麦芽酮糖醇的大鼠在断奶后4周，盲肠（充盈和排空状态）的相对重量增加。5%异麦芽酮糖醇组雄性大鼠充盈盲肠的相对重量也增加。这些发现并未伴有腹泻或盲肠的组织学变化。本研究结果未表明在连续三代中，给大鼠喂食高达10%饮食水平的异麦芽酮糖醇会对其繁殖、母体性能或发育产生任何有害影响。
