National Organization of Drug Control and Research, Cairo, Egypt.
Drug Test Anal. 2012 Feb;4(2):129-35. doi: 10.1002/dta.253. Epub 2011 Mar 4.
The catalytic effect of iodide on the oxidation of four dyes: viz. variamine blue (VB), methylene blue (MB), rhodamine B (RB), and malachite green (MG) with different oxidizing agents was investigated for the kinetic spectrophotometric determination of iodide. The above catalyzed reactions were monitored spectrophotometrically by following the change in dye absorbances at 544, 558, 660, or 617 nm for the VB, RB, MB, or MG catalyzed reactions, respectively. Under optimum conditions, iodide can be determined within the concentration levels 0.064-1.27 µg mL(-1) for VB method, 3.20-9.54 µg mL(-1) for RB method, 5.00-19.00 µg mL(-1) for the MB method, and 6.4-19.0 µg mL(-1) for the MG one, with detection limit reaching 0.004 µg mL(-1) iodide. The reported methods were highly sensitive, selective, and free from most interference. Applying the proposed procedures, trace amounts of iodide in pharmaceutical and edible salt samples were successfully determined without separation or pretreatment steps.
碘化物对四种染料（即变色酸蓝（VB）、亚甲蓝（MB）、罗丹明 B（RB）和孔雀石绿（MG））与不同氧化剂氧化的催化作用进行了研究，以用于动力学分光光度法测定碘化物。通过分别监测 VB、RB、MB 和 MG 催化反应中染料吸光度在 544、558、660 或 617nm 处的变化，对上述催化反应进行了分光光度监测。在最佳条件下，VB 法可在 0.064-1.27µg·mL(-1)的浓度范围内、RB 法在 3.20-9.54µg·mL(-1)的浓度范围内、MB 法在 5.00-19.00µg·mL(-1)的浓度范围内、MG 法在 6.4-19.0µg·mL(-1)的浓度范围内测定碘化物，检测限达到 0.004µg·mL(-1)。所报道的方法具有高灵敏度、高选择性且不受大多数干扰。应用所提出的程序，无需分离或预处理步骤，即可成功测定药物和食用盐样品中的痕量碘化物。
