Stafford C A, Shehab S A, Nona S N, Cronly-Dillon J R
Department of Optometry and Vision Sciences, UMIST, Manchester, England.
Glia. 1990;3(1):33-42. doi: 10.1002/glia.440030106.
By using an antibody to goldfish glial fibrillary acidic protein (GFAP), the reaction of goldfish optic nerve to injury has been studied by immunoblotting and immunohistochemical methods. Goldfish optic nerve, which normally lacks GFAP immunoreactivity (Nona et al.: Glia, 2:189-200, 1989), expresses GFAP following injury. This immunoreactivity, which is observed as early as 10 days after crush and which is still evident at 30 days after crush, all but disappears by 150 days after crush. Since it is well established that functional restoration of synaptic connections and the recovery of vision takes place in goldfish following optic nerve injury, our results indicate that reactive astrocytes do not represent an impediment to regeneration in goldfish visual system.
通过使用针对金鱼胶质纤维酸性蛋白（GFAP）的抗体，采用免疫印迹和免疫组织化学方法研究了金鱼视神经对损伤的反应。正常情况下缺乏GFAP免疫反应性的金鱼视神经（诺纳等人：《胶质细胞》，第2卷，第189 - 200页，1989年）在损伤后会表达GFAP。这种免疫反应性在挤压后10天就可观察到，在挤压后30天仍然明显，但在挤压后150天几乎消失。由于已经明确金鱼视神经损伤后突触连接的功能恢复和视力恢复会发生，我们的结果表明反应性星形胶质细胞并不代表金鱼视觉系统再生的障碍。
