Danilova T A, Gorskaia Iu F, Lunin V G, Grabko V I, Sharapova N E, Nesterenko V G
Zh Mikrobiol Epidemiol Immunobiol. 2010 Nov-Dec(6):52-7.
To assess effect of continued immunization of mice from CBA line with inactivated group A streptococcal vaccine on levels of serum cytokines and number of stromal bone marrow progenitor cells in immunized mice and in heterotopic transplants after different variants of transplantation.
CBA mice were immunized during 3 weeks with heat-killed vaccine prepared from group A streptococci type 5. Levels of pro- and antiinflammatory cytokines were measured with BioPlex device. Number of stromal progenitor cells was determined on quantity of colonies formed by cells explanted to monolayer cultures.
Significant 2.3-fold increase of number of stromal progenitor cells in femoral bone marrow of immune mice was demonstrated. Experiments with heterotopic transplants showed that bone marrow in transplants of mice immunized with streptococci--variant Normal-->Immune (N-->1)-- is defective both on efficacy of cloning and number of stromal progenitor cells. Even short-term presence of stromal tissue in immune organism (variant I -->N) significantly changed these parameters, especially at late time after transplantation. In serum of immune mice changes of cytokines levels, especially TNFalpha, were observed. The level of the latter was decreased (mean--2.5-fold) in all immune serum samples compared to normal serum.
Immunization of mice with group A streptococci leads to changes in stromal tissue and, possibly, to damage of microenvironment functions including hemo- and lymphopoiesis.
评估用灭活的A组链球菌疫苗持续免疫CBA系小鼠，对免疫小鼠及不同移植变体后异位移植中血清细胞因子水平和基质骨髓祖细胞数量的影响。
用从5型A组链球菌制备的热灭活疫苗对CBA小鼠进行为期3周的免疫。用BioPlex设备测量促炎和抗炎细胞因子的水平。通过接种到单层培养物中的细胞形成的集落数量来确定基质祖细胞的数量。
免疫小鼠股骨骨髓中基质祖细胞数量显著增加2.3倍。异位移植实验表明，用链球菌免疫的小鼠移植——正常→免疫（N→I）变体——在克隆效率和基质祖细胞数量方面均存在缺陷。即使免疫机体中基质组织短期存在（I→N变体）也会显著改变这些参数，尤其是在移植后的晚期。在免疫小鼠血清中观察到细胞因子水平的变化，尤其是肿瘤坏死因子α（TNFα）。与正常血清相比，所有免疫血清样本中TNFα水平均降低（平均降低2.5倍）。
用A组链球菌免疫小鼠会导致基质组织发生变化，并可能损害包括造血和淋巴细胞生成在内的微环境功能。
