P.O Box 2455, College of science, Department of Physics, King Saud University, Riyadh, 1145 Kingdom of Saudi Arabia.
Technol Cancer Res Treat. 2011 Apr;10(2):145-52. doi: 10.7785/tcrt.2012.500189.
This paper pertains to a new technique based on fluorescence emission spectra (FES), and stokes shift spectra (SSS) of blood plasma, acetone extract of cellular fraction, and urine. These samples were collected from 60 cancer patients of different etiology and 60 age adjusted controls for a single blind study. A set of ratio parameters were obtained from the above spectra (FES and SSS of above three sets of samples), based on the relative intensity of biofluorophores like tryptophan, tyrosine, flavin etc. It was found that these biofluorophores go out of proportion for malignancy of any etiolology. The study was done in two phases: calibration and validation. Based on a certain set of ratios obtained by simple statistical analysis, in the calibration phase, the blinded samples of validation phase were spectrally analysed and classified as normal or malignant. The scoring done by independent oncologists (who were not involved in any part of this new technique) yielded an overall sensitivity of 87%, and specificity of 83%. The result indicate that new optical spectroscopic techniques could be a simple, non-invasive protocol for detection of cancers, particularly in symptomatic cases; or for monitoring the post treated cases of cancer.
本文涉及一种基于荧光发射光谱（FES）和血桨、细胞部分丙酮提取物和尿液的Stokes 位移光谱（SSS）的新技术。这些样本取自 60 名不同病因的癌症患者和 60 名年龄匹配的对照者，进行单盲研究。从上述光谱（上述三组样本的 FES 和 SSS）中获得了一组比参数，基于色氨酸、酪氨酸、黄素等生物荧光团的相对强度。结果发现，这些生物荧光团的比例与任何病因的恶性肿瘤不成比例。该研究分为校准和验证两个阶段：基于通过简单统计分析获得的某一组比值，在校准阶段，对验证阶段的盲样进行光谱分析并分类为正常或恶性。由独立肿瘤学家（未参与本新技术的任何部分）进行的评分得出总体敏感性为 87%，特异性为 83%。结果表明，新的光学光谱技术可能是一种简单、非侵入性的癌症检测方案，特别是在有症状的病例中；或用于监测癌症治疗后的病例。
