采用高效液相色谱法（HPLC）与放射免疫分析法（RIA）联用测定尿液中的α-人心房钠尿肽（α-hANP），结果有力地表明存在非免疫反应性代谢产物。

Determination of alpha-human atrial natriuretic peptide (alpha-hANP) in urine using combination HPLC with RIA strongly indicates non-immunoreactive metabolites.

作者信息

Missbichler A, Hartter E, Woloszczuk W, Pittner F

机构信息

II. Med Univ. Klinik, Vienna.

出版信息

J Biochem Biophys Methods. 1990 Jan;20(2):113-21. doi: 10.1016/0165-022x(90)90070-s.

DOI:10.1016/0165-022x(90)90070-s

PMID:2138188

Abstract

Employing HPLC coupled with RIA, it was shown that alpha-human atrial natriuretic peptide is excreted in urine. Freshly collected urine had to be acidified to obtain reproducible results. When prepurified urine was subjected to HPLC (ion exchange and reversed phase) the subsequent quantification of alpha-hANP immunoreactive material in the eluate showed 10- to 30-fold greater amounts of alpha-hANP after treatment with HPLC; substances with the same elution parameters as synthetic alpha-hANP were detected, but they gave no response in the RIA.

摘要

采用高效液相色谱法（HPLC）结合放射免疫分析法（RIA），结果表明人α-心房钠尿肽在尿液中排泄。新鲜收集的尿液必须酸化才能获得可重复的结果。当对预纯化的尿液进行HPLC（离子交换和反相）分析时，洗脱液中α-hANP免疫反应性物质的后续定量显示，经HPLC处理后α-hANP的量增加了10至30倍；检测到与合成α-hANP具有相同洗脱参数的物质，但它们在RIA中无反应。