Togashi K, Hirata Y, Ando K, Takei Y, Kawakami M, Marumo F
Kitasato Biochemical Laboratories, Department of Physiology, Kitasato University School of Medicine, Kanagawa, Japan.
FEBS Lett. 1989 Jul 3;250(2):235-7. doi: 10.1016/0014-5793(89)80728-8.
A highly specific and sensitive radioimmunoassay (RIA) for a novel porcine brain natriuretic peptide (BNP) has been established to elucidate whether BNP-like immunoreactivity (LI) is present in human plasma. The antibody used was specific for BNP without any crossreactivities with known human atrial natriuretic peptides (hANP). After extraction of human plasma with Sep-Pak cartridge, this assay allowed for detection of BNP-LI as low as 0.1 fmol/tube. In 12 healthy subjects, the mean concentrations of plasma BNP-LI were 1.5 fmol/ml. Reverse-phase HPLC coupled with BNP RIA revealed that the single major component with BNP-LI, corresponding to porcine BNP(1 26), was apparently distinct from that of alpha-hANP. These data indicate that a small molecular mass form with BNP-LI circulates in human blood.
为了阐明人血浆中是否存在脑钠肽样免疫反应性（LI），已建立了一种针对新型猪脑钠肽（BNP）的高特异性和高灵敏度放射免疫分析（RIA）方法。所使用的抗体对BNP具有特异性，与已知的人心房钠尿肽（hANP）无任何交叉反应。用Sep-Pak柱提取人血浆后，该分析方法能够检测低至0.1 fmol/管的BNP-LI。在12名健康受试者中，血浆BNP-LI的平均浓度为1.5 fmol/ml。反相高效液相色谱结合BNP RIA显示，具有BNP-LI的单一主要成分，对应于猪BNP(1-26)，明显不同于α-hANP。这些数据表明，具有BNP-LI的小分子形式在人血液中循环。
