Omya Development AG, R&D-Microbiology, 4665 Oftringen, Switzerland.
Int J Cosmet Sci. 2011 Oct;33(5):426-31. doi: 10.1111/j.1468-2494.2011.00648.x. Epub 2011 Mar 8.
Given that a variety of bacterial species may occur in the calcium carbonate powder used for cosmetic formulations, an understanding of their diversity and abundance is necessary to accurately assess the contamination of the finished product. 16S rRNA was PCR-amplified from genomic DNA extracted from three different calcium carbonate powder grades, and these amplicon libraries were sequenced using deep amplicon sequencing technology. The resulting libraries contained 4149-6688 16S rRNA reads per sample with a length of 327-342 bp. Classification into genus of pyrosequencing reads of the dominant bacterial species found in calcium carbonate powders was used to confirm the absence of Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella spp. and Escherichia coli. The analysis described here can be used to determine the microbial diversity of calcium carbonate powder or the presence of any 'indicator microorganisms' in raw materials as well as in cosmetic products. This work provides guidance for prioritizing subsequent culturable and quantitative analysis, ensuring that potentially significant microorganisms are not left out of risk estimations.
鉴于化妆品配方中使用的碳酸钙粉末中可能存在多种细菌种类，因此有必要了解其多样性和丰度，以便准确评估成品的污染情况。从三种不同等级的碳酸钙粉末中提取基因组 DNA 后，对 16S rRNA 进行了 PCR 扩增，然后使用深度扩增子测序技术对这些扩增子文库进行了测序。每个样本的文库包含 4149-6688 条 16S rRNA 读长，长度为 327-342bp。对碳酸钙粉末中优势细菌种的焦磷酸测序读长进行分类，以确认无金黄色葡萄球菌、铜绿假单胞菌、沙门氏菌和大肠杆菌。这里描述的分析可用于确定碳酸钙粉末的微生物多样性，或原材料以及化妆品产品中任何“指示微生物”的存在。这项工作为后续可培养和定量分析提供了指导，确保在风险评估中不会遗漏潜在的重要微生物。
