Department of Orthopedics, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, China.
Biochem Biophys Res Commun. 2011 Apr 8;407(2):327-32. doi: 10.1016/j.bbrc.2011.03.015. Epub 2011 Mar 5.
The aim of this study was to construct tissue-engineered composite intervertebral disc (IVD) consisting of demineralized bone matrix gelatin (DBMG) and collagen II/hyaluronate/chondroitin-6-sulfate (CII/HyA-CS) scaffolds seeded with anulus fibrosus (AF) and nucleus pulposus (NP) cells, respectively. The cell-scaffold hybrids were implanted in the subcutaneous space of the dorsum of athymic mice and harvested at 4, 8, and 12 weeks. At each time point, the gross and histological morphology and biochemical properties were evaluated. Our results are as following: the gross morphology and histology of the composite resembled those of native IVD. Morphological studies revealed progressive tissue formation and junction integration between AF and NP regions. Biochemical composition detection indicated that the content of DNA, proteoglycan and hydroxyproline increased with time, and were similar to native tissue at 12 weeks. All these results demonstrated the feasibility of creating a tissue-engineered composite IVD with similar morphological and biochemical properties to the native tissue.
本研究旨在构建组织工程化复合椎间盘（IVD），由脱矿骨基质明胶（DBMG）和胶原 II/透明质酸/硫酸软骨素-6（CII/HyA-CS）支架组成，分别种植纤维环（AF）和髓核（NP）细胞。细胞-支架杂交体被植入无胸腺小鼠背部的皮下空间，并在 4、8 和 12 周时收获。在每个时间点，评估大体和组织形态学以及生化特性。我们的结果如下：复合组织的大体形态和组织学类似于天然 IVD。形态学研究显示，AF 和 NP 区域之间逐渐形成组织并进行连接整合。生化成分检测表明，DNA、糖胺聚糖和羟脯氨酸的含量随时间增加，12 周时与天然组织相似。所有这些结果表明，创建具有与天然组织相似的形态和生化特性的组织工程化复合 IVD 是可行的。
