通过构象转变将抗体固定在丝素蛋白表面。
Surface immobilization of antibody on silk fibroin through conformational transition.
机构信息
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, People's Republic of China.
出版信息
Acta Biomater. 2011 Jul;7(7):2782-6. doi: 10.1016/j.actbio.2011.03.001. Epub 2011 Mar 5.
In recent studies silk fibroin has been explored as a new material platform for biosensors. Based on these developments, a procedure for the immobilization of antibodies on silk fibroin substrates was developed as a route to functionalizing these biosensor systems. By controlling the conformational transition of the silk fibroin, a primary antibody was immobilized and enriched at the surface of silk fibroin substrates under mild reaction conditions to maintain antibody function. Compared to chemical crosslinking, the immobilization efficiency in the present approach was increased significantly. This method, achieving high loading of antibody while retaining function, improves the feasibility of silk fibroin as a platform material for biosensor applications.
在最近的研究中，丝素蛋白已被探索作为生物传感器的新材料平台。基于这些发展，开发了一种将抗体固定在丝素蛋白基质上的方法，作为使这些生物传感器系统功能化的途径。通过控制丝素蛋白的构象转变，在温和的反应条件下，将一种主要抗体固定和富集在丝素蛋白基质的表面上，以保持抗体的功能。与化学交联相比，本方法显著提高了抗体的固定效率。这种方法在保留功能的同时实现了抗体的高负载，提高了丝素蛋白作为生物传感器应用平台材料的可行性。
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