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肉和肉产品中产色胺的嗜热解糖梭菌分离株的体外合成及其他微生物的影响。

In vitro synthesis of biogenic amines by Brochothrix thermosphacta isolates from meat and meat products and the influence of other microorganisms.

机构信息

Technical University of Lodz, Institute of Fermentation Technology and Microbiology Wolczanska 171/173, 90-924 Lodz, Poland.

出版信息

Meat Sci. 2011 Jul;88(3):571-4. doi: 10.1016/j.meatsci.2011.02.015. Epub 2011 Feb 23.

DOI:10.1016/j.meatsci.2011.02.015
PMID:21382674
Abstract

Twenty Brochothrix thermosphacta strains tested for biogenic amines (BAs) production, formed histamine (6.6-16.2 mg/kg) and tyramine (18.7-35.4 mg/kg) but neither putrescine nor cadaverine. Six of the twenty strains were also investigated in respect of their influence on the synthesis of BAs by Escherichia coli, Pseudomonas sp., Proteus mirabilis and Lactobacillus sakei. In pure culture Escherichia coli produced all of the studied amines (histamine, tyramine, putrescine and cadaverine) with a total concentration of 167.7 mg/kg, P. mirabilis produced a total of 56.7 mg/kg histamine, tyramine and cadaverine, while Lactobacillus sakei and Pseudomonas sp. produced histamine and tyramine, totaling 37.9 and 35.2 mg/kg respectively. All B. thermosphacta promoted cadaverine production by Escherichia coli which increased by 12-68%, and some of them contributed to the appearance of this amine among the metabolites of Pseudomonas. The presence of B. thermosphacta decreased the potential ability of P. mirabilis to produce BAs.

摘要

20 株生孢食纤维单胞菌被测试用于生物胺(BAs)的生产,它们形成了组胺(6.6-16.2mg/kg)和酪胺(18.7-35.4mg/kg),但没有形成腐胺或尸胺。其中的 6 株生孢食纤维单胞菌还被研究了它们对大肠杆菌、假单胞菌、奇异变形杆菌和清酒乳杆菌合成 BAs 的影响。在纯培养中,大肠杆菌产生了所有研究的胺(组胺、酪胺、腐胺和尸胺),总量为 167.7mg/kg,奇异变形杆菌总共产生了 56.7mg/kg 的组胺、酪胺和尸胺,而清酒乳杆菌和假单胞菌产生了组胺和酪胺,总量分别为 37.9mg/kg 和 35.2mg/kg。所有生孢食纤维单胞菌都促进了大肠杆菌中尸胺的产生,使其增加了 12-68%,其中一些还导致了这种胺在假单胞菌代谢物中的出现。生孢食纤维单胞菌的存在降低了奇异变形杆菌产生 BAs 的潜在能力。

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