Thamsborg Kristian Key Milan, Lund Birgitte Winther, Byrne Derek Victor, Leisner Jørgen Johannes, Alexi Niki
Department of Veterinary and Animal Sciences, University of Copenhagen, Grønnegårdsvej 15, 1870 Copenhagen, Denmark.
Danish Technological Institute (DMRI), Gregersensvej 9, 2630 Taastrup, Denmark.
Foods. 2023 Dec 15;12(24):4489. doi: 10.3390/foods12244489.
This study investigated cadaverine as a spoilage indicator in commercial beef products stored under conditions favourable for the growth of lactic acid bacteria. Samples included vacuum-skin-packed entrecotes (EB) aged up to 42 days and modified-atmosphere-packed (70% O + 30% CO) minced beef (MB) stored at 5 °C. Two MB product lines were analysed: one stored aerobically two days post-slaughter before mincing and another stored for 14 days in vacuum packaging prior to mincing. Sensory assessment/evaluation and microbial analysis were performed throughout the shelf life of the products and compared to cadaverine levels measured using LC-MS/MS. Cadaverine concentrations in EB reached approximately 40,000 µg/kg on the "best before" date, while remaining below 50 µg/kg in both MB products on the corresponding date. While cadaverine concentrations in EB displayed a consistent increase, suggesting its potential as a spoilage indicator post-ageing, the low concentrations in MB, did not correlate with sensory assessments, revealing its limitations as a universal spoilage marker. In conclusion, it is necessary to conduct product-specific studies to evaluate the applicability of cadaverine as a spoilage indicator for beef products.
本研究调查了尸胺作为在有利于乳酸菌生长的条件下储存的商业牛肉产品腐败指标的情况。样本包括真空包装的肉眼牛排(EB),陈化长达42天,以及气调包装(70%氧气+30%二氧化碳)的碎牛肉(MB),储存于5°C。分析了两条MB产品线:一条在屠宰后有氧储存两天后切碎,另一条在切碎前真空包装储存14天。在产品的整个保质期内进行了感官评估和微生物分析,并与使用液相色谱-串联质谱法测定的尸胺水平进行了比较。EB中的尸胺浓度在“保质期前”日期达到约40,000微克/千克,而在相应日期,两种MB产品中的尸胺浓度均低于50微克/千克。虽然EB中的尸胺浓度呈现持续上升趋势,表明其作为陈化后腐败指标的潜力,但MB中的低浓度与感官评估不相关,揭示了其作为通用腐败标志物的局限性。总之,有必要进行针对特定产品的研究,以评估尸胺作为牛肉产品腐败指标的适用性。