Departamento de Biocatálisis, Instituto de Catálisis-CSIC, Campus UAM, Calle Marie Curie, 2, Cantoblanco, 28049 Madrid, Spain.
Colloids Surf B Biointerfaces. 2011 Jun 1;84(2):556-60. doi: 10.1016/j.colsurfb.2011.02.017. Epub 2011 Feb 16.
Sucrose laurate is a detergent that is useful for various biochemical applications because it is a green compound and is easily degradable after hydrolysis with a lipase or esterase. One problem observed in the process of sucrose laurate degradation is that most commercial detergent preparations are impure, necessitating the hydrolysis of all of the sucrose esters present in the preparation, all of them with detergent properties. In this article, a highly active catalyst, which is able to perform the hydrolysis of commercial sucrose laurate, is presented. The use of glyoxyl agarose preparations of a previously aminated Thermomyces lanuginosa lipase (TLL) enabled complete hydrolysis, in less than 30 min, of all of the compounds that comprise the mixture. In addition, this derivative is stable in the presence of 20% ethanol, which is necessary to prevent microbial contamination.
月桂酸蔗糖酯是一种去污剂，在各种生化应用中都很有用，因为它是一种绿色化合物，在脂肪酶或酯酶水解后很容易降解。在月桂酸蔗糖酯降解过程中观察到的一个问题是，大多数商业洗涤剂制剂不纯，需要水解制剂中存在的所有蔗糖酯，它们都具有洗涤剂性能。本文介绍了一种高效催化剂，它能够水解商业月桂酸蔗糖酯。使用先前氨化的Thermomyces lanuginosa 脂肪酶 (TLL) 的乙二醛琼脂糖制剂，能够在不到 30 分钟的时间内完全水解混合物中的所有化合物。此外，该衍生物在 20%乙醇存在下稳定，这是防止微生物污染所必需的。
