[加工食品中兽药残留的快速同时测定]

[Quick simultaneous determination of residual veterinary drugs in processed food].

作者信息

Yoshida Emiko, Shibuya Takahiro, Kurokawa Chieko, Inoue Yutaka, Yamamoto Yoshihiko, Miyazaki Motonobu

机构信息

Division of Life Science, Saitama City Institute of Health Science and Research, Saitama-shi, Ibaraki, Japan.

出版信息

Shokuhin Eiseigaku Zasshi. 2011;52(1):59-65. doi: 10.3358/shokueishi.52.59.

DOI:10.3358/shokueishi.52.59

PMID:21383536

Abstract

Development of rapid and high accuracy analysis, and tightening of regulations for veterinary drugs are required because many examples of detection of veterinary drugs in many kinds of processed food have been reported. In this study, we constructed an improved method for simultaneous determination of veterinary drugs, based on the glass bead homogenization method with EDTA-2Na and batch purification for QuEChERS analysis. Our extraction procedure is suitable for handling multiple samples quickly and easily. Furthermore, our improved extraction solvent allowed simultaneous determination of tetracyclines in processed food. In a test of 69 veterinary drugs, recovery of over 60 ranged from 70 to 120%, with a coefficient of variation of less than 25% and with quantification limits of 0.01 µg/g (S/N≥10 ). This improved method is expected to be useful for quick simultaneous determination of multiple residues.

摘要

由于已报道了多种加工食品中兽药检测的诸多实例，因此需要开发快速且高精度的分析方法，并加强兽药监管。在本研究中，我们基于使用乙二胺四乙酸二钠（EDTA - 2Na）的玻璃珠匀浆法和用于QuEChERS分析的批量净化法，构建了一种改进的兽药同时测定方法。我们的提取程序适用于快速、轻松地处理多个样品。此外，我们改进的提取溶剂能够同时测定加工食品中的四环素。在对69种兽药的测试中，回收率超过60%的范围为70%至120%，变异系数小于25%，定量限为0.01 µg/g（S/N≥10）。这种改进的方法有望用于多种残留的快速同时测定。