Ghaffari Ali R, Noshad Hamid, Ostadi Ali, Hasanzadeh Nazila
Department of Internal Medicine, Toxicology and Nephrology Ward, Tuberculosis and Lung Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Saudi Med J. 2011 Mar;32(3):249-53.
To evaluate the effectiveness of cyclophosphamide and methylprednisolone pulse therapy in the prevention of pulmonary fibrosis due to paraquat poisoning in rats.
This study was carried out in Sina Hospital, Tabriz, Iran, between February and August 2009. Acute poisoning in rats was induced by intraperitoneal injection of Paraquat (15 mg/kg). We planned 2 separate treatment groups (10 rats each), pulse therapy with methylprednisolone (26.5 mg/kg) and cyclophosphamide (15 mg/kg) intraperitoneally for 3 days (PQ+P) and treatment with conventional anti-oxidant drugs including vitamin A, vitamin E, and N-acetylcysteine (PQ+Vit). Prevention of pulmonary fibrosis was evaluated on the fifteenth day. A semi-quantitative determination of lung fibrosis was carried out (Ashcroft staging criteria) on the lung sections and results compared with the paraquat control group (PQ).
The mean score of fibrosis in the PQ was 4.60+/-1.20, in PQ+P was 2.93+/-0.72, and for PQ+Vit groups was 4.25+/-1.08. The score of fibrosis in the PQ+P was significantly lower than the PQ group (p=0.011), while there was no significant difference in the average score of lung fibrosis between the PQ and PQ+Vit groups.
Pulse therapy with cyclophosphamide and methylprednisolone significantly prevented pulmonary fibrosis. Therefore, we recommend it along with conventional therapies in the treatment of acute paraquat poisoning.
评估环磷酰胺和甲泼尼龙脉冲疗法对预防大鼠百草枯中毒所致肺纤维化的有效性。
本研究于2009年2月至8月在伊朗大不里士的新浪医院进行。通过腹腔注射百草枯（15毫克/千克）诱导大鼠急性中毒。我们计划了2个单独的治疗组（每组10只大鼠），腹腔内注射甲泼尼龙（26.5毫克/千克）和环磷酰胺（15毫克/千克）进行脉冲治疗3天（PQ+P），以及用包括维生素A、维生素E和N-乙酰半胱氨酸在内的传统抗氧化药物进行治疗（PQ+Vit）。在第15天评估肺纤维化的预防情况。对肺切片进行肺纤维化的半定量测定（阿什克罗夫特分期标准），并将结果与百草枯对照组（PQ）进行比较。
PQ组的纤维化平均评分为4.60±1.20，PQ+P组为2.93±0.72，PQ+Vit组为4.25±1.08。PQ+P组的纤维化评分显著低于PQ组（p=0.011），而PQ组和PQ+Vit组之间的肺纤维化平均评分无显著差异。
环磷酰胺和甲泼尼龙脉冲疗法可显著预防肺纤维化。因此，我们建议在急性百草枯中毒的治疗中，将其与传统疗法联合使用。
