Department of Mechanical Engineering, Bio/Nanofluidics Lab, Chosun University, Gwangju 501-759, Korea.
J Biomed Mater Res B Appl Biomater. 2011 May;97(2):263-70. doi: 10.1002/jbm.b.31809. Epub 2011 Mar 7.
Biomedical scaffolds used in bone tissue engineering should have various properties including appropriate bioactivity, mechanical strength, and morphologically optimized pore structures. Collagen has been well known as a good biomaterial for various types of tissue regeneration, but its usage has been limited due to its low mechanical property and rapid degradation. In this work, a new hybrid scaffold consisting of polycaprolactone (PCL) and collagen is proposed for bone tissue regeneration. The PCL enhances the mechanical properties of the hybrid scaffold and controls the pore structure. Layered collagen nanofibers were used to enhance the initial cell attachment and proliferation. The results showed that the hybrid scaffold yielded better mechanical properties of pure PCL scaffold as well as enhanced biological activity than the pure PCL scaffold did. The effect of pore size on bone regeneration was investigated using two hybrid scaffolds with pore sizes of 200 ± 20 and 300 ± 27 μm. After post-seeding for 7 days, the cell proliferation with pore size, 200 ± 20 μm, was greater than that with pore size, 300 ± 27 μm, due to the high surface area of the scaffold.
用于骨组织工程的生物医学支架应具有多种性能，包括适当的生物活性、机械强度和形态优化的孔隙结构。胶原蛋白作为各种组织再生的良好生物材料已广为人知，但由于其机械性能低和降解迅速，其应用受到限制。在这项工作中，提出了一种由聚己内酯 (PCL) 和胶原蛋白组成的新型混合支架用于骨组织再生。PCL 增强了混合支架的机械性能并控制了孔隙结构。分层的胶原纳米纤维用于增强初始细胞附着和增殖。结果表明，与纯 PCL 支架相比，混合支架具有更好的机械性能和增强的生物活性。通过使用孔径为 200 ± 20 和 300 ± 27 μm 的两种混合支架研究了孔径对骨再生的影响。接种后 7 天，由于支架的高表面积，孔径为 200 ± 20 μm 的细胞增殖大于孔径为 300 ± 27 μm 的细胞增殖。
