Blackstone Britani N, Gallentine Summer C, Powell Heather M
Department of Materials Science and Engineering, The Ohio State University, Columbus, OH 43210, USA.
Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA.
Bioengineering (Basel). 2021 Mar 18;8(3):39. doi: 10.3390/bioengineering8030039.
Collagen is a key component of the extracellular matrix (ECM) in organs and tissues throughout the body and is used for many tissue engineering applications. Electrospinning of collagen can produce scaffolds in a wide variety of shapes, fiber diameters and porosities to match that of the native ECM. This systematic review aims to pool data from available manuscripts on electrospun collagen and tissue engineering to provide insight into the connection between source material, solvent, crosslinking method and functional outcomes. D-banding was most often observed in electrospun collagen formed using collagen type I isolated from calfskin, often isolated within the laboratory, with short solution solubilization times. All physical and chemical methods of crosslinking utilized imparted resistance to degradation and increased strength. Cytotoxicity was observed at high concentrations of crosslinking agents and when abbreviated rinsing protocols were utilized. Collagen and collagen-based scaffolds were capable of forming engineered tissues in vitro and in vivo with high similarity to the native structures.
胶原蛋白是全身各器官和组织细胞外基质（ECM）的关键组成部分，在许多组织工程应用中都有使用。静电纺丝胶原蛋白可以生产出各种形状、纤维直径和孔隙率的支架，以匹配天然细胞外基质。本系统综述旨在汇总现有关于静电纺丝胶原蛋白和组织工程的手稿数据，以深入了解原材料、溶剂、交联方法和功能结果之间的联系。在使用从实验室中经常分离的小牛皮中分离出的I型胶原蛋白形成的静电纺丝胶原蛋白中，最常观察到D带，溶解时间短。所有使用的物理和化学交联方法都赋予了抗降解性并提高了强度。在高浓度交联剂和使用简略冲洗方案时观察到细胞毒性。胶原蛋白和基于胶原蛋白的支架能够在体外和体内形成与天然结构高度相似的工程组织。
