Bioorganic Chemistry Division, Indian Institute of Integrative Medicine (CSIR), Jammu and Kashmir, India.
J Sep Sci. 2011 Apr;34(7):749-60. doi: 10.1002/jssc.201000718. Epub 2011 Mar 7.
High-performance thin layer chromatography (HPTLC) method for the separation and quantitative determination of ten markers (catechins, flavonoids, and phenolics) in different extracts of green tea and guava leaf has been developed and the antioxidant activity profiles of the two plant extracts have been determined. Ten marker compounds have been resolved using silica gel 60 F(254) plates, toluene/acetone/formic acid (5:4:1 v/v/v) for markers 1-6, and toluene/ethyl acetate/formic acid/methanol (3:3:0.8:0.2 v/v/v/v) for markers 7-10 as the mobile phases. The high-performance thin layer chromatography densitometry was performed at wavelengths of 282 and 285 nm for the markers 1-6 and 7-10, respectively. Potent antioxidant activity and the presence of phenolics and flavan-3-ols has been observed for the guava leaf extracts suggestive of its use as an alternate economical source of antioxidants over green tea--the well-established food additive/nutraceutical agent.
已开发出一种高效薄层色谱（HPTLC）方法，用于分离和定量测定绿茶和番石榴叶不同提取物中的十种标记物（儿茶素、类黄酮和酚类），并测定了这两种植物提取物的抗氧化活性谱。使用硅胶 60 F（254）板，甲苯/丙酮/甲酸（5:4:1 v/v/v）分离标记物 1-6，甲苯/乙酸乙酯/甲酸/甲醇（3:3:0.8:0.2 v/v/v/v）分离标记物 7-10 作为流动相。标记物 1-6 的高效薄层色谱分光光度法在 282 和 285nm 波长下进行，标记物 7-10 的高效薄层色谱分光光度法在 282 和 285nm 波长下进行。番石榴叶提取物具有很强的抗氧化活性和酚类及黄烷-3-醇类物质的存在，表明其可用作绿茶的替代经济抗氧化剂来源——这是一种成熟的食品添加剂/营养保健品。
