School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa, Japan.
Macromol Biosci. 2011 Jun 14;11(6):765-71. doi: 10.1002/mabi.201000507. Epub 2011 Mar 7.
Functionalized polyrotaxanes are utilized to investigate the relation to multivalent interactions between the mannose moiety and Con A immobilized surfaces. According to the results of SPR spectroscopy, the mannose-conjugated polyrotaxanes show a higher response than any other mannose conjugate on both surfaces of high- and low-density Con A. Moreover, the results of the FRET analysis suggest that the mobility of α-cyclodextrins in the polyrotaxane more efficiently contributes to their binding interactions in a multivalent manner. This well-defined polyrotaxane system provides control over ligand density, ligand mobility, and gives an efficient response to the biological interaction receptor, which has not been easy to achieve in covalently bound polymeric systems.
功能化聚轮烷用于研究甘露糖部分与固定化 ConA 之间的多价相互作用关系。根据 SPR 光谱学的结果，与高和低密度 ConA 的两种表面上的任何其他甘露糖缀合物相比，甘露糖-聚轮烷具有更高的响应。此外，FRET 分析的结果表明，聚轮烷中α-环糊精的迁移率以多价方式更有效地促进其结合相互作用。这种明确的聚轮烷体系可以控制配体密度和配体迁移率，并对生物相互作用受体做出有效响应，这在共价键合聚合物体系中不容易实现。
