一种用于检测ATP酶活性的连续荧光测定法。
A continuous fluorimetric assay for ATPase activity.
作者信息
Banik U, Roy S
机构信息
Department of Biophysics, Bose Institute, Calcutta, India.
出版信息
Biochem J. 1990 Mar 1;266(2):611-4.
Abstract
A new continuous coupled fluorimetric assay is described for ATPases in general. Thus phosphate released from ATP hydrolysis is coupled to the nucleoside phosphorylase reaction using 7-methylguanosine as a fluorescent substrate for the nucleoside phosphorylase reaction. The hydrolysis of 7-methylguanosine leads to 7-methylguanine, which has lower quantum yield and hence can be used to monitor ATP hydrolysis continuously. The method has the potential to be extended to GTPase and nucleotidyltransferase assays.
摘要
本文描述了一种全新的用于一般ATP酶的连续偶联荧光测定法。因此，ATP水解释放的磷酸盐与核苷磷酸化酶反应相偶联，使用7-甲基鸟苷作为核苷磷酸化酶反应的荧光底物。7-甲基鸟苷的水解产生7-甲基鸟嘌呤，其量子产率较低，因此可用于连续监测ATP水解。该方法有可能扩展到GTP酶和核苷酸转移酶的测定。
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