Ecole Polytechnique Fédérale de Lausanne, Institut des Matériaux and Institut des Sciences et Ingénierie Chimiques, Laboratoire des Polymères Bâtiment MXD, Station 12, CH-1015 Lausanne, Switzerland.
ACS Comb Sci. 2011 May 9;13(3):286-97. doi: 10.1021/co100099r. Epub 2011 Mar 8.
This manuscript describes a versatile, yet experimentally facile, method for producing libraries of polymer-coated (core−shell type) gold nanoparticles. The synthetic principle relies on two, sequential postmodification reactions, which ensures homogeneity across each series. First, poly(pentafluorophenyl methacrylate) synthesized by RAFT polymerization is used here as a reactive precursor, which can be modified, postpolymerization, to create a library of functional polymers each bearing a ω-thiol end-group. In a second step, these well-defined polymers are then tethered by their ω-thiol group to the surface of prefabricated citrate-stabilized gold nanoparticles to give a library of 75 unique, yet sequentially modified organic−inorganic hybrid particles. The optical properties of the gold core were exploited to create a high-throughput assay for investigating the role of nanoparticle size and surface coating on aggregation in various biologically relevant media. These experiments demonstrated the importance of the type of dissolved salts present and also the strong influence of serum proteins in cell-culture media and their interactions with nanoparticles surfaces, which in turn might affect their biological profiles. Therefore, this method presents a powerful, yet accessible tool for creating model nanoparticle libraries with intrinsic sensing properties.
本文描述了一种通用且实验简便的方法，用于制备聚合物包覆（核壳型）金纳米粒子库。该合成原理依赖于两个连续的后修饰反应，可确保每个系列的均一性。首先，本文使用通过 RAFT 聚合合成的聚（五氟苯基甲基丙烯酸酯）作为反应性前体，其可在聚合后进行修饰，以创建一系列带有 ω-巯基端基的功能聚合物。在第二步中，这些结构明确的聚合物通过其 ω-巯基基团被键合到预制的柠檬酸稳定的金纳米粒子表面，得到 75 种独特的、但顺序修饰的有机-无机杂化粒子。金核的光学性质被利用来创建高通量测定法，以研究纳米粒子尺寸和表面涂层对在各种生物相关介质中聚集的作用。这些实验证明了存在的溶解盐的类型以及细胞培养介质中的血清蛋白的强烈影响及其与纳米粒子表面的相互作用，这反过来可能影响它们的生物学特性。因此，该方法提供了一种强大且易于使用的方法，用于创建具有固有传感特性的模型纳米粒子库。
