Food Industry Research and Development Institute, Hsinchu, Taiwan.
J Agric Food Chem. 2011 Apr 13;59(7):3078-86. doi: 10.1021/jf105012v. Epub 2011 Mar 18.
Sesame seeds (Sesamum indicum L.) are unique because of potent and various physiological activities imparted by their bioactive lignans. This investigation studied the intestinal distribution and excretion of sesaminol in Sprague-Dawley (SD) rats. To investigate the distribution of sesaminol (per oral 220 mg/kg), the changes in concentration of sesaminol and its metabolites were determined in the intestines and plasma within the 24 h period after tube feeding of sesaminol to SD rats. Results show that the epimerization of sesaminol appeared to be catalyzed by acid in the simulated gastric fluids. The major sesaminol epimer was characterized as 2-episesaminol using 2D-NMR. These findings indicate that sesame sesaminol and its epimer are poorly absorbed prior to reaching the rectum and that substantial amounts pass from the small to the large intestine, where they are metabolized by the colonic microflora to tetrahydrofuranoid metabolites. Sesaminol in plasma was largely present as phase II conjugates, and the seven metabolites were detected as the 2-episesaminol, sesaminol-6-catechol, methylated sesaminol-catechol, R,R-hydroxymethylsesaminol-tetrahydrofuran, S,R-hydroxymethylsesaminol-tetrahydrofuran, enterolactone, and enterodiol. Excretions of sesaminol in urine and feces within the 24 h period were equivalent to 0.02 and 9.33% of the amount ingested, respectively.
芝麻籽（Sesamum indicum L.）因其生物活性木脂素赋予的强大而多样的生理活性而独具特色。本研究探讨了芝麻素在 Sprague-Dawley（SD）大鼠肠道中的分布和排泄。为了研究芝麻素（口服 220mg/kg）的分布情况，在 SD 大鼠经管饲给予芝麻素后 24 小时内，测定了肠道和血浆中芝麻素及其代谢物的浓度变化。结果表明，芝麻素的差向异构化似乎是在模拟胃液中的酸催化下发生的。主要的芝麻素差向异构体被鉴定为 2-episesaminol，使用二维 NMR 进行鉴定。这些发现表明，芝麻素在到达直肠之前吸收不良，大量从小肠转移到大肠，在那里被结肠微生物群代谢为四氢呋喃型代谢物。血浆中的芝麻素主要以 II 期结合物的形式存在，检测到 7 种代谢物，分别为 2-episesaminol、sesaminol-6-catechol、methylated sesaminol-catechol、R,R-hydroxymethylsesaminol-tetrahydrofuran、S,R-hydroxymethylsesaminol-tetrahydrofuran、enterolactone 和 enterodiol。在 24 小时内，芝麻素在尿液和粪便中的排泄量分别相当于摄入量的 0.02%和 9.33%。
